The dandruff was under the electron microscope, and no abnormality was found.
The big stone in Zhou Wen’s heart dropped two-thirds.
However, he will not be completely relieved until the mutant aphid is tested tomorrow and it is confirmed that there is no virus.
After the busy work here, it just happened to be lunch time.
Zhou Wen came to Shi Lei and asked with a smile, “Brother Lei, do you need help?”
Shi Lei, who had just prepared the electrophoresis gel and was about to kill the guinea pig, said without raising his head, “What’s the matter, are you finished?”
Zhou Wen said with a smile: “I’m so busy, just go back and change the data, you know.”
It usually takes 2 to 3 days for an electrophoresis process to run down, plus PCR must be done. After two or three days of hard work, it is often found that the experimental data is seriously unreasonable.
And then there’s f*ck, Jesus, damnit, all roaring.
Next, if you don’t plan to do it all over again, you can only fake the data.
This is basically an unspoken rule for biological students, and most biological students have done this.
Shi Lei laughed twice, he was also a visitor, nodded and said, “If that’s the case, then you can go back to rest first, I’ll do it myself. By the way, help us with a meal by the way.”
Zhou Wen turned around, leaned on the test bench and said, “Why are you going back, I don’t have a girlfriend.”
Wearing a white lab coat with her back to Lin Jiayi, who was looking at the microscope, she turned around and said, “Ai Zhou Wen, do you still have contact with Qu Fangfang?”
Qu Fangfang turned out to be the prettiest among the girls in the lab. She has a gentle personality and a small family. She is the ideal wife for boys.
It’s a pity that I went for an internship after the year.
Zhou Wen replied: “I haven’t contacted, what’s the matter?”
“It’s nothing.” Lin Jiayi said casually, followed by giggling: “Ai Zhou Wen, you invite me to dinner, how about me being your girlfriend?”
Zhou Wen curled his lips and said, “As long as you women have a word to say, my child will now play soy sauce.”
Shi Lei laughed.
Lin Jiayi also turned around with a smile.
It’s just a joke. She has already studied second year. She knows exactly what kind of boyfriend she should find.
Here, Zhou Wen turned his head and smiled: “Seriously, Brother Lei, do you want to help? If not, I’ll go back and watch the drama.”
As soon as Shi Lei heard that he was going back to watch dramas, he raised his head and smiled and said, “Then you should stay, just as Lan Lan asked me to go to the movies in the afternoon.”
Zhou Wen urged: “Then why don’t you hurry back and get your hands dirty, and try to successfully intubate at night.”
Shi Lei smiled naively.
Similar to Zhou Wen, Shi Lei’s family conditions are not very good. In addition, he looks like a blind monk, and he doesn’t speak sweet words. He is almost graduating from graduate school, and he has never even kissed a **** the lips.
It’s rare to meet a girl who doesn’t dislike him, and everyone is very happy for him.
The receiving plate is also recognized.
“Then I’m leaving?”
“Go.”
Shi Lei gave Zhou Wen the guinea pig in his hand, turned and left the laboratory.
“Remember to bring us meals.”
“Know…”
Here, Zhou Wen pinched the mouse’s neck with one hand and pressed it on the console, while the other hand grabbed the mouse’s tail and lifted it up. With a “click”, he gave the mouse a “click”. Cervical vertebra decapitation”.
No pain at all.
Then quickly disemboweled, cut off the femur of the mouse with scissors, and peeled off the muscles and fascia outside the leg bone.
After rinsing, cut the femur, extract the bone marrow, then rinse the femoral bone marrow with hypotonic solution, and finally put the rinsed “rinsing solution” into a test tube in equal parts for each strain.
Then, a mouse was strangled to death. After extracting the bone marrow, the rinsing solution was prepared step by step according to the above steps, and the previous rinsing solution was prepared according to the weight of the AB group, and then the centrifuge began to centrifuge.
The genes of mice are 95% similar to human genes, so many large laboratories will use the genes of mice for various drug tests.
However, it usually takes 2 to 3 days for a gene extraction process to complete. Those large laboratories and research institutes that pay attention to efficiency will hand over the gene extraction work to laboratories in major universities.
A mouse can theoretically extract 4 to 8 gene fragments, but normally only 4 strains can be extracted.
Of course, these are for a fee.
The current market price of a plant is about 400~500.
…
Just after the centrifugation was completed, Shi Lei came back with the packed meal.
After having dinner in the office outside, Zhou Wen was going to do electrophoresis analysis, commonly known as “running electrophoresis”.
This has already been initially contacted in high school.
It is to use agarose gel to make a gel plate, so that the sample is electrophoresed on the gel plate, so it is also called “running gel”, which can be used to detect DNA strands.
In addition to running electrophoresis, another method is electron microscope observation, which saves much time compared to running electrophoresis.
However, for electron microscope observation, glass slides need to be prepared, and genes need to be stained. During this process, time, technique, temperature, and possible contamination may cause the extraction to fail, and all previous efforts will be wasted.
So novices generally run electrophoresis honestly.
Zhou Wen took the extraction gun and prepared to extract the supernatant and then “beat”. At this moment, he suddenly thought of “the eye of true vision”.
Want to see how the Eye of True Sight introduced DNA?
He raised the test tube in his right hand and shook it twice against the light in front of the operating table. At the same time, his eyes narrowed slightly, staring at the liquid in the centrifuge tube.
What Zhou Wen couldn’t believe was that the image of the DNA strand of the white mouse appeared directly in front of his eyes, including the broken covalent bond.
“My Nima…”
Zhou Wen was so excited that he almost jumped up.
His eyes can see DNA clearly, doesn’t that mean his eyes have caught up with electron microscopes?
He immediately put down the centrifuge tube in his hand, took off the rubber gloves and spread his hands, his eyes narrowed slightly, imagining the “Eye of True Sight” in his mind.
With a “swoosh”, his hand was as clear as a glass slide under a microscope.
But the next second, he couldn’t be happy, and he saw Staphylococcus, Staphylococcus aureus and long E. coli in his palms…
There are all kinds of other bacteria and microbes crawling around in the palm of your hand.
In addition, the inside of the nails is like a nest of bacteria, filled with all kinds of bacteria, which looks very disgusting.
I’ve washed my hands. I don’t know how many germs there will be if I don’t wash my hands?
“Fuck~” Zhou Wen was so frightened that he shook his hands twice, and then he remembered that germs could not be thrown away, so he hurried to wash his hands again.
He knew that people had a lot of bacteria on their hands, but they were all made with bacteria and then observed under a microscope.
Because the human hand is opaque, there is no way to see it directly.
Because of this, he has never had such an intuitive visual shock and realized the importance of hand washing for the first time.
Zhou Wen rolled up his sleeves, first washed his hands with alcohol-based hand sanitizer, then found a nail clipper, carefully trimmed the dirty nails, and then washed his hands carefully again.
Now look at ~IndoMTL.com~ with the eyes of true sight ~IndoMTL.com~ The bacteria on the hands have been eliminated by more than 90%, and only a small number of stubborn bacteria are still attached to the fingers and skin folds.
Zhou Wen said speechlessly: “It’s no wonder that experiments often fail. It’s no wonder that the success rate is high because of the number of bacterial groups in hand.”
Returning to the laboratory, I looked at the rubber gloves on the table with True Sight, and found that they were also covered with bacteria.
“Huh~” Zhou Wen picked up the vaccination stick on the table next to him, picked up the gloves and threw them into the waste bin, and then found a pair of sterile gloves to put on.
After everything was done again, Zhou Wen excitedly picked up the group A centrifuge tube containing the mouse gene fragment and observed it again.
Not surprisingly, a clear DNA image appeared in front of him.
This shows that the gene extraction of the mice was successful.
The purpose of electrophoresis and electron microscope observation is to detect whether there is DNA in the test tube.
Now he can see the DNA directly, which can be said to have saved the most cumbersome and easily failed procedure.
Then he picked up the centrifuge tube of group B and looked at it again, but this time he didn’t see the DNA strand.
Zhou Wen secretly said: “Could it be that the extraction failed?”
After putting it down, he picked up another tube of clear liquid from group B, squinted his eyes and looked, but still couldn’t see the DNA strand.
He picked up one of the tubes from Group A again, but this time he saw it clearly.
This also shows that Group B really failed to extract.
“Obviously all the processes are exactly the same, I don’t know which link went wrong?” Zhou Wen frowned and pondered.
He decided to do an electrophoresis analysis first to see if what the True Sight saw was real, and then to study the errors in the operation…
——
ps: I know I’m wrong, please forgive me.